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  • 328R-1 - PU.1 (EPR3158Y) Rabbit Monoclonal Primary Antibody

328R-1 Sigma-Aldrich

PU.1 (EPR3158Y) Rabbit Monoclonal Primary Antibody

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Cell Marque Antibodies, Hematopathology, PT-PY,
conjugate   unconjugated
clone   EPR3158Y, monoclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
packaging   vial of 0.1 mL concentrate (328R-14)
  vial of 0.5 mL concentrate (328R-15)
  bottle of 1.0 mL predilute (328R-17)
  vial of 1.0 mL concentrate (328R-16)
  bottle of 7.0 mL predilute (328R-18)
antibody form   culture supernatant
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
description   For In Vitro Diagnostic Use in Select Regions (See Chart)
mfr. no.   Cell Marque
concentration   0.01-1.0 μg/mL (predilute)
  0.4-40 μg/mL (concentrate)
control   tonsil
visualization   nuclear


General description

PU.1 is a transcription factor that has been shown to be important for normal B-cell development. PU.1 belongs to the ETS family of transcription factors. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. Very little is known about PU.1 function in later stages of B-cell development. PU.1 does not seem to play a role in the end-stage of B-cell development and is not expressed in plasma cells. PU.1 exerts an important role in the regulation of the expression of crucial B-cell proteins, such as immunoglobulin (Ig) genes, and CD20 and its putative binding sites were also identified in the promoters of CD79, CD10, and CD22. PU.1 binds to the 3’ enhancer region of both the Ig kappa and lambda light chain genes and it also regulates the immunoglobulin heavy chain genes through the intron enhancer region. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker including the following: B-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, and nodular lymphocyte predominant Hodgkin lymphoma.

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC







PU.1 Positive Control Slides, Product No. 328S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Antibody Basics

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328S PU.1 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) 

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