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  • 345R-1 - Uroplakin III (SP73) Rabbit Monoclonal Antibody

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345R-1 Sigma-Aldrich

Uroplakin III (SP73) Rabbit Monoclonal Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Cell Marque Antibodies, Genitourinary (GU) Pathology, Primary Antibodies,
conjugate   unconjugated
clone   SP73, monoclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500-1:1000
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
packaging   vial of 0.1 mL concentrate (345R-14)
  vial of 0.5 mL concentrate (345R-15)
  bottle of 1.0 mL predilute (345R-17)
  vial of 1.0 mL concentrate (345R-16)
  bottle of 7.0 mL predilute (345R-18)
antibody form   culture supernatant
isotype   IgG
antibody product type   primary antibodies
Quality Level   GMP-IVD
description   For In Vitro Diagnostic Use in Select Regions (See Chart)
mfr. no.   Cell Marque
concentration   0.001-0.05 μg/mL (predilute)
  0.8-40 μg/mL (concentrate)
control   bladder
visualization   membranous

Description

General description

Uroplakins (UPs) are a family of transmembrane proteins (UPs Ia, Ib, II and III) that are specific differentiation products of urothelial cells. In non-neoplastic mammalian urothelium, UPs are expressed in the luminal surface plasmalemma of superficial (umbrella) cells, forming complexes of 16nm crystalline particles. Anti-UPIII was detectable immunohistochemically in 29 of 55 primary (53%) and 23 of 35 metastatic (66%) urothelial carcinomas, whereas many non-urothelial carcinomas were anti-UPIII negative. The authors concluded that anti-UPIII should be a valuable marker, especially for the specific identification of urothelial carcinomas in patients with metastases of unknown primary site. Olsburgh et al. studied anti-UPIII gene expression in normal urothelium and bladder cancer specimens, and found that expression was absent after malignant transformation. Thus, UP expression might reflect the malignant potential of urothelial cancer cells as well as being cytodifferential markers of urothelial cells. Ohtsuka et al. concluded in their studies that anti-UPIII expression was strongly associated with lower tumor grades, that lack of UPIII expression in urothelial tumors of the upper urinary tract was associated with much higher rates of metastases, and that 5-year specific survival was much worse for anti- UPIII negative tumors (54%) than for anti-UPIII positive tumors (100%). Apparently, UPIII expression characteristics are a better indicator of the malignant potential of urothelial carcinomas than the grade of these tumors.

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

Linkage

Uroplakin III Positive Control Slides, Product No. 345S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

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345S Uroplakin III Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) 

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