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  • 35848 - 2′,7′-Dichlorofluorescein

35848 Sigma-Aldrich


BioReagent, suitable for fluorescence, ≥90% (T)

  • CAS Number 76-54-0

  • Empirical Formula (Hill Notation) C20H10Cl2O5

  • Molecular Weight 401.20

  •  Beilstein/REAXYS Number 58009

  •  EC Number 200-968-6

  •  MDL number MFCD00005047

  •  PubChem Substance ID 57649439



Related Categories Alphabetical, Biochemicals and Reagents, Fluorescent Indicators and Probes, Fluorescent Probes, Labels, Particles and Stains, pH Sensitive Probes and Indicators More...
product line   BioReagent
assay   ≥90% (T)
mp   280 °C (dec.) (lit.)
fluorescence   λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0
suitability   suitable for fluorescence



2′,7′-Dichlorofluorescein diacetate may be used as a superior gas phase alternative to underivatized fluorescein and as a component/substrate of the 2′,7′-dichlorofluorescein diacetate assay to quantitate reactive oxygen species (ROS).

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What can be used to make a solution of 2´,7´-dichlorofluorescein?
2´,7´-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2´,7´-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.
In what types of applications can 2´,7´-Dichlorofluorescein be used?
Some applications for use of 2´,7´-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC.See G. Gbitz et al., J. Chromatogr., 117, 337, (1976). 2´,7´-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as afluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).
What excitation /emission wavelengths are best for fluorescence detection of 2´,7´-Dichlorofluorescein?
2´,7´-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.
How do I handle autoradiography film?
Please view autoradiography film tips provided by Carestream Health, Inc.
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