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365M-9 Sigma-Aldrich

PNL2 (PNL2) Mouse Monoclonal Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Cell Marque Antibodies, Dermatopathology, PJ-PN,
Quality Level   GMP-IVD
biological source   mouse
antibody form   culture supernatant
antibody product type   primary antibodies
clone   PNL2, monoclonal
description   For In Vitro Diagnostic Use in Select Regions (See Chart)
form   buffered aqueous solution
species reactivity   human
packaging   vial of 0.1 mL concentrate (365M-94)
  vial of 0.5 mL concentrate (365M-95)
  bottle of 1.0 mL predilute (365M-97)
  vial of 1.0 mL concentrate (365M-96)
  bottle of 7.0 mL predilute (365M-98)
mfr. no.   Cell Marque
concentration   0.01-1 μg/mL (predilute)
  0.8-2.5 μg/mL (concentrate)
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
isotype   IgG1
conjugate   unconjugated
control   melanoma
shipped in   wet ice
storage temp.   2-8°C
visualization   cytoplasmic

Description

General description

Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. Anti-PNL2 yields a strong cytoplasmic staining of skin and oral mucosal melanocytes, and staining of granulocytes when used at high concentration. Anti-PNL2 labels intraepidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, Melan-A and HMB-45 stain most clear cell sarcoma cells and a few cells in angiomyolipomas and lymphangioleiomyomatosis. Non-melanocytic lesions found to be positive with this marker include PEComas and melanotic schwannoma. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB-45, MART-1, Tyrosinase, and MiTF. The differential diagnosis between benign nevi and malignant melanoma is sometimes difficult, and malignant melanoma may mimic other neoplasms, such as undifferentiated carcinoma, sarcoma or large cell lymphoma. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87%), as opposed to 76% for anti-HMB-45 and 82% for anti-MART-1. Furthermore, anti-PNL2, combined with antibodies against S-100 and HMB-45 is a highly sensitive and specific marker for mucosal melanoma.

Linkage

PNL2 Positive Control Slides, Product No. 365S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Preparation Note

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Quality


IVD

IVD

IVD

RUO

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Immunohistochemistry: Current Applications in Skin Cancer

Cutaneous Spindle Cell Tumors Cutaneous Adnexal Neoplasms Melanocytic Neoplasms Malignant Small Blue Cell Tumors Fibrohistiocytic Lesions Vascular Neoplasms Hematopoietic and Hystiocytic Tumors Infec...
Mike Lacey
M.D. Pathologist and Chief Medical Officer
Keywords: AGE, Addition reactions, Adhesion, Cancer, Cell biology, Clinical, Condensations, Diagnostic, Diseases, Gastrointestinal, Gene expression, Genetic, Growth factors, Immunohistochemistry, Immunostaining, Indicators, Infectious Diseases, Inflammation, Microarray Analysis, Microscopy, Polymerase chain reaction, Techniques and applications, Tissue microarrays, Transcription

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Peer-Reviewed Papers
15

References

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365S PNL2 Positive Control Slides, immunohistochemistry (formalin-fixed, paraffin-embedded sections) 

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