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371R-1 Sigma-Aldrich

SOX-2 (SP76) Rabbit Monoclonal Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Cell Marque Antibodies, Primary Antibodies, Pulmonary Pathology,
Quality Level   GMP-IVD
biological source   rabbit
antibody form   culture supernatant
antibody product type   primary antibodies
clone   SP76, monoclonal
description   For In Vitro Diagnostic Use in Select Regions (See Chart)
form   buffered aqueous solution
species reactivity   human
packaging   vial of 0.1 mL concentrate (371R-14)
  vial of 0.5 mL concentrate (371R-15)
  bottle of 1.0 mL predilute (371R-17)
  vial of 1.0 mL concentrate (371R-16)
  bottle of 7.0 mL predilute (371R-18)
mfr. no.   Cell Marque
concentration   0.01-1.0 μg/mL (predilute)
  0.5-50 μg/mL (concentrate)
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200
isotype   IgG
conjugate   unconjugated
control   squamous epithelium
shipped in   wet ice
storage temp.   2-8°C
visualization   nuclear

Description

General description

It has been reported that anti-SOX-2 antibody recognizes lung squamous cell carcinoma (LSCC). A recent study has demonstrated that extensive anti-SOX-2 staining was seen in over 90% of LSCC and largely paralleled p63 expression. Extensive anti-SOX-2 staining was seen in 21% of lung adenocarcinomas (LACA), including cases that were anti-p63-negative or only anti-p63 focally-positive. However, another recent study showed only 4.5% of LACA is positive for anti-SOX-2 expression. In a study by Sholl et al, 29% of LACA cases exhibited at least focal p63 expression. Combined p63 and SOX-2 expression was seen in 94% of LSCC and 12% of LACA with a statistically significant difference (P<0.0001) versus p63 alone. This study also showed that anti-CK5/6 had a good sensitivity but poor specificity for LSCC. Combined anti-CK5/6 and anti-p63 positivity was seen in 93% of LSCC and 24% of LACA. Anti-CK5/6+/anti-p63+/anti- SOX-2+ was detected in 93% of LSCC and only 9% of LACA. These results indicate that the sensitivity of anti-p63 is equally high but its specificity is similarly variable; it was seen at least focally in close to 30% of LACA. When used together, anti-p63+/anti-SOX-2+ applied to the same tumor cell population is >90% specific for LSCC. Anti-SOX-2 produced moderate-to-intense staining in all 50 cases of embryonal carcinoma components. The only other component that showed reactivity was the primitive neuroectodermal component in 11 of 14 (79%) of immature teratomas. In each of these positive staining foci, the staining varied from moderate-to-strong. Yolk sac tumor, seminoma, mature teratoma, choriocarcinoma, and IGCNU were uniformly negative, as were all the non-neoplastic parenchymal and stromal structures.

Linkage

SOX-2 Positive Control Slides, Product No. 371S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Preparation Note

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Quality


IVD

IVD

IVD

RUO

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
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References

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371S SOX-2 Positive Control Slides, immunohistochemistry (formalin-fixed, paraffin-embedded sections) 

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