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37291 Sigma-Aldrich

Dihydroethidium

BioReagent, suitable for fluorescence, ≥95% (HPCE)

Synonym: 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine

  • CAS Number 104821-25-2

  • Empirical Formula (Hill Notation) C21H21N3

  • Molecular Weight 315.41

  •  Beilstein/REAXYS Number 5107482

  •  MDL number MFCD00077335

  •  PubChem Substance ID 57649715

  •  NACRES NA.32

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Properties

Related Categories Biochemicals and Reagents, Detection, Fluorescent Enzyme Substrates, Fluorescent Enzyme Substrates for Flow Cytometry, Fluorescent Enzyme Substrates for Microscopy,
Quality Level   100
product line   BioReagent
assay   ≥95% (HPCE)
solubility   acetonitrile: soluble
  methanol: soluble
fluorescence   λex 358 nm; λem 461 nm
  λex 392 nm; λem 410 nm in methanol
suitability   suitable for fluorescence
storage temp.   −20°C
SMILES string   CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14
InChI   1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
InChI key   XYJODUBPWNZLML-UHFFFAOYSA-N

Description

Application

Dihydroethidium (dHEt), a fluorescene probe, is used to detect the presence of reactive oxygen species (ROS) such as superoxides.

Redox indicator. Blue fluorescence until oxidized to ethidium.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Linkage

B-ring reduced analog of ethidium

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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