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38733 Sigma-Aldrich

Laemmli Lysis-buffer

non smelling

  •  NACRES NA.25

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Properties

Related Categories Buffers, Buffers and Reagents, Buffers, Reagents, & Stains, Core Bioreagents, General Reagents,
Quality Level   100
form   liquid
composition   bromophenol blue, 0.004%
  DTT, 400 mM
  glycerol, 20%
  SDS, 4%
  TRIS, 0.125 M
pH   ~6.8
suitability   in accordance for electrophoresis test
storage temp.   −20°C

Description

Application

Laemmli Lysis-buffer is an non-smelling lysis buffer used to prepare protein samples for SDS gel electrophoresis.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the difference between the two Laemmli buffers S3401 and 38733?
Product No. S3401 is 0.125M Tris (pH 6.8) in 80% water/20% glycerol, with 4% SDS, 10% 2-mercaptoethanol and 0.004% bromophenol blue. 38733 is 0.125M Tris in 80% water/20% glycerol, with 4% SDS, 400 mM DTT and 0.004% bromophenol blue.So the only difference is the reducing agent; 2-mercaptoethanol (strong odor) vs. the DTT (not nearly as strong an odor).
What is the advantage of using Laemmli Lysis-buffer,Product No. 38733?
The odor of 38733 is not nearly as strong as that of S3401 (which has 2-mercaptoethanol).Some people are very sensitive to the odor of 2-mercaptoethanol.
Is Laemmli Lysis-buffer, Product No. 38733, a 1× or a 2× solution?
For most applications, it is considered to be a 2X solution.
Why do the Laemmlisample buffers like S3401 and 38733 have glycerol in them?
An aqueous buffer with 20% glycerol is denser than one without. So when the sample is added to the well of an electrophoresis gel, it tends to stay in the well.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Buffers - From Bnech to Bulk
Protocols & Articles
Peer-Reviewed Papers
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