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  • 389M-1 - Glutamine Synthetase (GS-6) Mouse Monoclonal Antibody

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389M-1 Sigma-Aldrich

Glutamine Synthetase (GS-6) Mouse Monoclonal Antibody

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Cell Marque Antibodies, GD-GL, Gastrointestinal (GI) Pathology,
conjugate   unconjugated
clone   GS-6, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
packaging   vial of 0.1 mL concentrate (389M-14)
  vial of 0.5 mL concentrate (389M-15)
  bottle of 1.0 mL predilute (389M-17)
  vial of 1.0 mL concentrate (389M-16)
  bottle of 7.0 mL predilute (389M-18)
antibody form   culture supernatant
isotype   IgG2a
Quality Level   100
  500
antibody product type   primary antibodies
description   For In Vitro Diagnostic Use in Select Regions (See Chart)
concentration   0.01-1 μg/mL (predilute)
  2.5-10 μg/mL (concentrate)
control   hepatocellular carcinoma
visualization   cytoplasmic

Description

General description

Glutamine synthetase (GS) catalyzes the synthesis of glutamine from glutamate and ammonia in the mammalian liver.1 In normal liver, GS expression is seen in pericentral hepatocytes, but not in mid-zonal or periportal hepatocytes.1 Glutamine, the end product of GS activity, is the major energy source of tumor cells.1 Based on findings from experimental hepatocarcinogenesis, GS positive tumor cells are believed to be derived from GS positive hepatocytes.1 Thus, anti-GS has been suggested as a marker for hepatocellular carcinoma (HCC).1 GS immunoreactivity has been seen in a majority of HCC (37 of 53 cases, 70%), including 7 of 10 cases of early HCC (70%) and 12 of 22 (59%) for low grade HCC.1 In nonmalignant nodules, GS overexpression was only seen in 3 high grade dysplastic nodules (HGDN,13.6%). In these cases, GS overexpression was restricted to 11.5%-50% of hepatocytes, whereas in HCC the majority of cases (28 of 53, 53%), including early HCC (60%), showed diffuse immunostaining (>50% tumor cells). Overall, the sensitivity, specificity, and positive and negative predictive values of anti-GS for HCC detection were 69.8%, 94.2%, 92.5%, and 75.4%, respectively. A panel composed of antibodies against HSP70, GPC3, and GS has been proposed to be very useful in distinguishing between dysplastic and early malignant hepatocellular nodules arising in cirrhosis. The “all positive” phenotype is restricted to approximately half of early HCC to well-differentiated HCC but has never been reported in dysplastic lesions, whereas the reverse phenotype, “all negative”, has been shown to be a feature of the majority of HGDN and of all low grade dysplastic nodules.

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Quality


IVD

IVD

IVD

RUO

Linkage

Glutamine Synthetase Positive Control Slides, Product No. 389S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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389S Glutamine Synthetase Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) 

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