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393A-1 Sigma-Aldrich

Adipophilin Rabbit Polyclonal Antibody

  •  NACRES NA.41

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Properties

Related Categories AD-AD, Alphabetical Index, Antibodies, Cell Marque Antibodies, Dermatopathology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
packaging   vial of 0.1 mL concentrate (393A-14)
  vial of 0.5 mL concentrate (393A-15)
  bottle of 1.0 mL predilute (393A-17)
  vial of 1.0 mL concentrate (393A-16)
  bottle of 7.0 mL predilute (393A-18)
antibody form   Ig fraction of antiserum
Quality Level   100
  500
antibody product type   primary antibodies
description   For In Vitro Diagnostic Use in Select Regions (See Chart)
mfr. no.   Cell Marque
concentration   0.01-1 μg/mL (predilute)
  2.5-10.0 μg/mL (concentrate)
control   sebaceous neoplasms (intracytoplasmic lipid droplet)
visualization   membranous

Description

General description

Sebaceous carcinoma is a relatively uncommon cutaneous malignancy and mimics other malignant neoplasms, such as basal and squamous cell carcinomas, and benign processes, such as chalazions and blepharitis, sometimes resulting in delayed diagnosis and suboptimal treatment.Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various normal cell types. Recently, it has been reported that adipophilin was expressed in 16 of 16 (100%) sebaceous adenomas with a specific pattern: membranous with strong uptake at the periphery of intracytoplasmic lipid vacuoles.

Of 25 sebaceous carcinomas, 23 (92%) were also labeled with a similar pattern. Additionally, in cases of poorly differentiated sebaceous carcinoma (n=11), in which sebaceous differentiation could not have been reliably interpreted in H&E sections, adipophilin highlighted the sebocytes with a strong membranous labeling of intracytoplasmic lipid droplets in 9 of 11 cases (82%). Moreover, 10 of 10 (100%) xanthelasmas, 9 of 10 (90%) xanthogranulomas, 6 of 6 (100%) xanthomas, and 9 of 13 (63%) metastatic renal cell carcinomas were also weakly-to- moderately positive for adipophilin. Expression of adipophilin with a membranous pattern of staining was not seen in any of the other clear cell lesions of the skin, including basal and squamous cell carcinomas, trichilemmomas, clear cell hidradenomas, or balloon cell nevi. Interestingly, a nonspecific granular uptake of anti-adipophilin was seen in adjacent macrophages, keratohyalin granules of epithelial squamous cells, and some tumor cells. Therefore, this anti-adipophilin is suitable for immunostaining formalin-fixed, paraffin-embedded tissue and is helpful in the identification of intracytoplasmic lipids, as seen in sebaceous lesions. It is especially helpful in identifying intracytoplasmic lipid vesicles in poorly differentiated sebaceous carcinomas in challenging cases such as small periocular biopsy specimens.

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

Quality


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Linkage

Adipophilin Positive Control Slides, Product No. 393S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

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M.D. Pathologist and Chief Medical Officer
Keywords: AGE, Addition reactions, Adhesion, Cancer, Cell biology, Clinical, Condensations, Diagnostic, Diseases, Gastrointestinal, Gene expression, Genetic, Growth factors, Immunohistochemistry, Immunostaining, Indicators, Infectious Diseases, Inflammation, Microarray Analysis, Microscopy, Polymerase chain reaction, Techniques and applications, Tissue microarrays, Transcription

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Peer-Reviewed Papers
15

References

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393S Adipophilin Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) 

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