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402RA-05A Sigma-Aldrich

Human Chondrocytes - Rheumatoid Arthritis: HC-RA, adult

  •  NACRES NA.81

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Properties

Related Categories Cell Culture, Chondrocytes, Primary Cell Culture, Primary Cells
Quality Level   100
biological source   human articular cartilage (rheumatoid arthritic)
packaging   pkg of 500,000 cells
growth mode   Adherent
karyotype   2n = 46
morphology   chondrocyte
application(s)   cell culture | mammalian: suitable
relevant disease(s)   arthritis
shipped in   dry ice
storage temp.   −196°C

Description

General description

HC-RA Human Chondrocytes are derived from human articular cartilage of donors with rheumatoid arthritis (RA). Rheumatoid arthritis is an inflammatory disease that is characterized by overproliferation of synovial cells and their infiltration into adjacent joint tissues, accompanied by gradual destruction of cartilage and bone erosion. Normally, chondrocytes are responsible for producing and maintaining the extracellular matrix of cartilage, but in RA cartilage, the environment is changed significantly due to inflammation and the invasion of other cells. Therefore, HC-RA can be a useful model to study the effects of those changes on chondrocyte biology. In parallel with normal HC, HC-RA can be used to investigate the differences between normal and pathological joints and evaluate potential treatment strategies.

Characterization: Positive for aggrecan after differentiation.

Cell Line Origin

Cartilage

Application

model to study effects of RA on chondrocytes, investigate differences between normal and pathological joints and evaluate potential treatment strategies

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

• 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 10 doublings

Subculture Routine

Please refer to the HC-RA Culture Protocol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Human Chondrocytes Rheumatoid Arthritis (HC-RA) Culture Protocol

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