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  • 40734 - Anti-Mouse IgG−Abberior® STAR 635 antibody produced in goat

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40734 Sigma-Aldrich

Anti-Mouse IgG−Abberior® STAR 635 antibody produced in goat

for STED application

Synonym: Abberior® STAR 635-Anti-Mouse-IgG antibody produced in goat

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Properties

Related Categories Alphabetical Index, Antibodies, MO-MR, Primary Antibodies
clone   polyclonal
biological source   goat
species reactivity   mouse
form   buffered aqueous solution
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   secondary antibodies
availability   not available in USA and Canada
concentration   ~1 mg/mL
fluorescence   λex 635 nm; λem 655 nm in PBS, pH 7.4

Description

General description

Abberior STAR 635 is a dye developed for STED and confocal imaging in the red spectral region. It features exceptional photostability, very high brightness and low background labeling. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. Abberior STAR 635 can substitute dyes like Atto 647N, Alexa Fluor® 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 635 (PBS pH 7.4), 635 (H2O), 635 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 110 000 (PBS pH 7.4), 110 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260max: 0,26 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,38 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 656 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,88 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 2,8 (PBS pH 7.4)

Application

Anti-Mouse IgG-abberior® STAR 635 antibody produced in goat has been used as a dye to mark the synaptic contacts.

abberior® STAR 635 particularly well suits the Abberior Instruments 2-color 775nm STED microscope and the Leica TCS STED system. It delivers high-resolution STED images with very low background signal due to its improved water solubility and exceptional brightness. The dye serves as an ideal partner for several 2-color STED packages.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Atto is a trademark of Atto-Tec GmbH

Cy is a registered trademark of GE Healthcare

abberior is a registered trademark of Abberior GmbH

Suitability

Designed and tested for fluorescent super-resolution microscopy

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Other Notes

Find more infomation here Abberior®

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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30558 Abberior® STAR 635, NHS ester, for STED application
96013 Abberior® STAR 635, maleimide, for STED application
30972 Abberior® STAR 635, phalloidin, for STED application
30483 Anti-Mouse IgG−Abberior® CAGE 635 antibody produced in goat, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
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