• USA Home
  • 41348 - Anti-Rabbit IgG−Abberior® STAR 635 antibody produced in goat

EMAIL THIS PAGE TO A FRIEND
41348 Sigma-Aldrich

Anti-Rabbit IgG−Abberior® STAR 635 antibody produced in goat

for STED application

Synonym: Abberior® STAR 635-Anti-Rabbit IgG antibody produced in goat

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RAB-RAD
antibody product type   primary antibodies
fluorescence   λex 633 nm; λem 655 nm±10 nm in PBS
storage temp.   −20°C

Description

Analysis Note

unconjugated dye ≤5% of total fluorescence;
may contain significant amounts of BSA as stabilizer

Application

Abberior STAR 635 particularly well suits the Abberior Instruments 2-color 775nm STED microscope and the Leica TCS STED system. It delivers high-resolution STED images with very low background signal due to its improved water solubility and exceptional brightness. The dye serves as an ideal partner for several 2-color STED packages.

General description

Abberior STAR 635 is the latest dye development for STED imaging in the red spectral region. It features exceptional brightness and ultralow background, delivering stunning STED images. The dye works exceptionally well with the Abberior Instruments STED microscope as well as with the Leica STED microscope.
Abberior STAR 635 can substitute ATTO 647N, Alexa Fluor® 647, or Cy®5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength around 750-780 nm is recommended.

Best results are obtained with freshly prepared samples.

Absorption Maximum, λmax: 635 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 110,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.26 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.38 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 656 nm (aq. acetonitrile; MeOH)
655 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 750 −780 nm
Fluorescence Quantum Yield, η: 0.88 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 2.8 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 2.8 ns (PBS, pH 7.4)

Other Notes

Find more infomation here Abberior®

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Atto is a trademark of Atto-Tec GmbH

Cy is a registered trademark of GE Healthcare

abberior is a registered trademark of Abberior GmbH

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary and Secondary Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay needs. Use the Antibody...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

30558 Abberior® STAR 635, NHS ester, for STED application
96013 Abberior® STAR 635, maleimide, for STED application
30972 Abberior® STAR 635, phalloidin, for STED application
40734 Anti-Mouse IgG−Abberior® STAR 635 antibody produced in goat, for STED application
30483 Anti-Mouse IgG−Abberior® CAGE 635 antibody produced in goat, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
53647 Anti-Rabbit IgG−Abberior® CAGE 635 antibody produced in goat, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?