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415M-1 Sigma-Aldrich

EZH2 (11) Mouse Monoclonal Antibody

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Anatomic Pathology, Antibodies, Cell Marque Antibodies, EO-ER,
conjugate   unconjugated
clone   11, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
packaging   vial of 0.1 mL concentrate (415M-14)
  vial of 0.5 mL concentrate (415M-15)
  bottle of 1.0 mL predilute (415M-17)
  vial of 1.0 mL concentrate (415M-16)
  bottle of 7.0 mL predilute (415M-18)
antibody form   culture supernatant
isotype   IgG1
Quality Level   100
  500
antibody product type   primary antibodies
description   For In Vitro Diagnostic Use in Select Regions (See Chart)
mfr. no.   Cell Marque
concentration   0.5-1.5 μg/mL (predilute)
  12.5-37.5 μg/mL (concentrate)
control   breast carcinoma, prostate adenocarcinoma, tonsil
visualization   nuclear

Description

General description

Enhancer of zeste homolog 2 (EZH2) is the catalytic subunit of polycomb repressive complex 2 (PRC2). It generates a methylation epigenetic mark at lysine 27 residue of histone H3 (H3K27me3) in order to silence gene expression. EZH2 target genes are involved in a variety of biological processes such as stem cell pluripotency, cell proliferation, and oncogenic transformation. EZH2 is frequently overexpressed in many cancer types. Hyperactivation of EZH2, either by overexpression or muta­tions, is found in a variety of malignancies including prostate, breast, uterine, gastric, and renal cell cancers in addition to melanoma. EZH2 overexpression has been reported in non-small cell lung cancers and lymphoma.The EZH2 protein is rarely detected in normal breast duct epithelium and in normal and hyperplastic lymph node. EZH2 is usually expressed in follicular centers, but not in mantle zones, follicular and interfollicular T cells, plasma cells or NK/T cells. However, its expression can be seen in most B-cell and T-cell lymphomas, although only in 20% (1/5) of small lymphocytic lymphoma and 14% (1/7) of plasma cell myeloma. Plasmacytoma, lymphoplasmacytic lymphoma, and MALT lymphoma have not been shown to express this protein. Recent studies also have demonstrated EZH2 is aberrantly over-expressed in pediatric rhabdomyosarcoma, independent of the histological subtypes. In summary, EZH2 correlates with tumor proliferation and may be used in an antibody panel to differentiate proliferative/aggressive lymphoma variants from indolent ones and normal resting cell populations

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

Quality


IVD

IVD

IVD

RUO

Linkage

EZH2 Positive Control Slides, Product No. 415S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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415S EZH2 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) 

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