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41639 Sigma-Aldrich

Dimethyl sulfoxide

BioUltra, for molecular biology, ≥99.5% (GC)

Synonym: DMSO

  • CAS Number 67-68-5

  • Linear Formula (CH3)2SO

  • Molecular Weight 78.13

  •  Beilstein/REAXYS Number 506008

  •  EC Number 200-664-3

  •  MDL number MFCD00002089

  •  PubChem Substance ID 57650076

  •  NACRES NA.25

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Properties

Related Categories Apoptosis and Cell Cycle, BioUltra, Cell Biology, Cell Signaling and Neuroscience, Molecular Biology,
Quality Level   200
grade   for molecular biology
vapor density   2.7 (vs air)
vapor pressure   0.42 mmHg ( 20 °C)
product line   BioUltra
assay   ≥99.5% (GC)
form   liquid
autoignition temp.   573 °F
expl. lim.   42 %, 63 °F
impurities   Dnases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
  ≤0.001% free acid (as CH3COOH)
evapn. residue   ≤0.005%
color   colorless
refractive index   n20/D 1.479 (lit.)
  n20/D 1.479
bp   189 °C (lit.)
mp   16-19 °C (lit.)
solubility   H2O: miscible (completely)
density   1.10 g/mL (lit.)
cation traces   Al: ≤0.5 mg/kg
  Ba: ≤0.1 mg/kg
  Bi: ≤0.1 mg/kg
  Ca: ≤0.5 mg/kg
  Cd: ≤0.05 mg/kg
  Co: ≤0.02 mg/kg
  Cr: ≤0.02 mg/kg
  Cu: ≤0.02 mg/kg
  Fe: ≤0.5 mg/kg
  K: ≤0.5 mg/kg
  Li: ≤0.1 mg/kg
  Mg: ≤0.1 mg/kg
  Mn: ≤0.02 mg/kg
  Mo: ≤0.1 mg/kg
  Na: ≤1 mg/kg
  Ni: ≤0.5 mg/kg
  Pb: ≤0.1 mg/kg
  Sr: ≤0.1 mg/kg
  Zn: ≤0.1 mg/kg
SMILES string   CS(C)=O
λ   neat
UV absorption   λ: 280 nm Amax: 0.22
suitability   suitable for molecular biology
InChI   1S/C2H6OS/c1-4(2)3/h1-2H3
InChI key   IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Description

General description

Dimethyl sulfoxide (DMSO) is an effective aprotic solvent. It is colorless, odorless and hygroscopic in nature. DMSO enhance the permeability of various hydrophilic and lipophilic permeants. It is one of the widely used solvents in the laboratory.

Application

Dimethyl sulfoxide (DMSO) has been used as a vehicle for test compounds in various experimental studies. It has also been used as a solvent during amyloid β (Aβ) aggregation protocol for resolubilization of peptide to maintain its dissociated state.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Other Notes

Polybrene / DMSO-assisted gene transfer

Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
WGK Germany 
WGK 1
RTECS 
PV6210000
Flash Point(F) 
188.6 °F - closed cup
Flash Point(C) 
87 °C - closed cup

Frequently Asked Questions

What is the molarity of DMSO?
DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.
What is the DMSO dissolved in?
DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.
Where does DMSO come from?
DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.
Does DMSO have any synthetic utility?
DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Dimethyl sulfoxide (DMSO) should be a liquid. Why did it arrive as a solid?
DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.
What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)?
DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is Sure/Seal packaging?
The Aldrich Sure/Seal packaging system is useful in the handling and storage of air-sensitive reagents.
Is Dimethyl sulfoxide a sterile product?
No, this product is not considered sterile.Product Nos. D2560 and D2438 are sterile-filtered.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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