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  • 41699 - Anti-Rabbit IgG−Abberior® STAR RED antibody produced in goat

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41699 Sigma-Aldrich

Anti-Rabbit IgG−Abberior® STAR RED antibody produced in goat

for STED application

Synonym: Abberior® STAR RED-Anti-Rabbit IgG antibody produced in goat

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RAB-RAD
clone   polyclonal
biological source   goat
species reactivity   rabbit
form   buffered aqueous solution
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   secondary antibodies
concentration   ~1 mg/mL
fluorescence   λex 638 nm; λem 655 nm in PBS, pH 7.4

Description

General description

Abberior STAR RED is a well-known red dye, named also KK114 in literature. It was developed for STED and confocal imaging in the red spectral region. It shows an exceptional brightness and low background, delivering stunning STED images. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. Abberior STAR RED can substitute dyes like Atto 647N, Alexa Fluor® 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Abberior STAR RED is the ideal partner for Abberior STAR 580 to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 125 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260max: 0,16 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,32 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 654 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,4 (PBS pH 7.4)

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Atto is a trademark of Atto-Tec GmbH

Cy is a registered trademark of GE Healthcare

abberior is a registered trademark of Abberior GmbH

Features and Benefits

• Unmatched, background free STED imaging cont
• Verified in Abberior Instruments and Leica STED microscopes

Suitability

Designed and tested for fluorescent super-resolution microscopy

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Other Notes

Find more infomation here Abberior®

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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Description

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43354 Abberior® STAR RED NHS, ≥80.0% (degree of coupling)
52283 Anti-Mouse IgG−Abberior® STAR RED antibody produced in goat, for STED application

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