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43816 Sigma-Aldrich

DL-Dithiothreitol solution

BioUltra, for molecular biology, ~1 M in H2O

Synonym: (±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT

  • CAS Number 3483-12-3

  • Empirical Formula (Hill Notation) C4H10O2S2

  • Molecular Weight 154.25

  •  Beilstein/REAXYS Number 1719757

  •  MDL number MFCD00004877

  •  PubChem Substance ID 57650196

  •  NACRES NA.25

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Properties

Related Categories Alphabetical, BioUltra, Biochemicals and Reagents, Core Bioreagents, Denaturation,
Quality Level   200
grade   for molecular biology
product line   BioUltra
concentration   ~1 M in H2O
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
pH   3.0-5.0
density   1.04 g/mL at 20 °C
absorption   cut-off at 330 nm
suitability   in accordance for filter test
storage temp.   2-8°C
SMILES string   O[C@H](CS)[C@H](O)CS
InChI   1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key   VHJLVAABSRFDPM-QWWZWVQMSA-N

Description

Application

DL-dithiothreitol solution has been used:
• in the treatment of intestinal explants
• in homogenate preparation of gill cells for enzymatic assays
• in western blotting
• in transglutaminase activity

Biochem/physiol Actions

DL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms. It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine residues positioned in the GABA(B1(a)) receptor structure.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Hand-casting gels for PAGE and SDS-PAGE using TurboMix™ Bis-Tris Gel Casting Kits

Polyacrylamide gel electrophoresis (PAGE) is a foundational technique used to separate proteins and other macromolecules by their electrophoretic mobility. Polyacrylamide gels are formed through the ...
Keywords: Alkylations, Cell disruption, Deaminations, Degradations, Electrophoresis, Gel electrophoresis, Nucleic acid denaturation, PAGE, Polymerization reactions, Sample preparations, Separation, Titrations

Peer-Reviewed Papers
15

References

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