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45245 Sigma-Aldrich

Eosin 5-isothiocyanate

≥95.0% (UV)

Synonym: EITC

  • CAS Number 60520-47-0

  • Empirical Formula (Hill Notation) C21H7Br4NO5S

  • Molecular Weight 704.96

  •  MDL number MFCD00082502

  •  PubChem Substance ID 57650268

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Probes, Labels, Particles and Stains, Functional Group Reactive Labels, Functional Group Reactive Labels for Microscopy, Luminescent Compounds/Detection,
Quality Level   100
assay   ≥95.0% (UV)
fluorescence   λex 521; λem 544 (pH 9.0)
storage temp.   2-8°C
SMILES string   OC(=O)c1cc(ccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c(O)c(Br)cc24)N=C=S
InChI   1S/C21H7Br4NO5S/c22-12-4-10-14(8-2-1-7(26-6-32)3-9(8)21(29)30)11-5-13(23)18(28)16(25)20(11)31-19(10)15(24)17(12)27/h1-5,27H,(H,29,30)
InChI key   MCXYFYWNOWPDMA-UHFFFAOYSA-N

Description

Application

Eosin 5-isothiocyanate (EITC) is a fluorescence probe. EITC tagged molecules may be used together with fluorescein-5′-isothiocyanate (FITC) tagged molecules in fluoresce resonance energy transfer (FRET) based assays.

Other Notes

Acceptor fluorophore. Measurement of rotational diffusion of proteins; In fluorescence energy transfer studies

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
Keywords: Applications, Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Methods, Microscopy, Neurotransmission, Sol-gel, Type

Peer-Reviewed Papers
15

References

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