• USA Home
  • 453R-1 - p53 (SP5) Rabbit Monoclonal Antibody

EMAIL THIS PAGE TO A FRIEND
453R-1 Sigma-Aldrich

p53 (SP5) Rabbit Monoclonal Antibody

  •  NACRES NA.41

Purchase

Properties

Related Categories Alphabetical Index, Anatomic Pathology, Antibodies, Cell Marque Antibodies, P-PA,
conjugate   unconjugated
clone   SP5, monoclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
packaging   vial of 0.1 mL concentrate (453R-14)
  vial of 0.5 mL concentrate (453R-15)
  bottle of 1.0 mL predilute (453R-17)
  vial of 1.0 mL concentrate (453R-16)
  bottle of 7.0 mL predilute (453R-18)
antibody form   culture supernatant
isotype   IgG1
Quality Level   500
antibody product type   primary antibodies
description   For In Vitro Diagnostic Use in Select Regions (See Chart)
mfr. no.   Cell Marque
concentration   0.01-1 μg/mL (predilute)
  4-11 μg/mL (concentrate)
control   colon carcinoma
visualization   nuclear

Description

General description

Anti-p53 tumor suppressor protein antibody recognizes a 53 kDa phosphoprotein, identified as p53 suppressor gene product. It reacts with the mutant as well as wild type p53, although significant accumulation of the mutant form of p53 protein due to longer half-life half-life is the basis for the test using the IHC technique. Quantitation of nuclear staining with this antibody has been shown to be a factor in breast carcinoma, lung carcinoma, colorectalcarcinoma, urothelial carcinoma, and ependymoma. p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

Linkage

p53 Positive Control Slides, Product No. 453S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

control

Product #

Description

Add to Cart

453S p53 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) 

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?