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46940 Sigma-Aldrich

6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester

suitable for fluorescence, ≥75% (HPLC)

Synonym: Fluorescein-5(6)-carboxamidocaproic acid N-succinimidyl ester

  • CAS Number 114616-31-8

  • Empirical Formula (Hill Notation) C31H26N2O10

  • Molecular Weight 586.55

  •  MDL number MFCD00467542

  •  PubChem Substance ID 329757270

  •  NACRES NA.32

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Properties

Related Categories Biochemicals and Reagents, Detection, Fluorescein and Derivatives, Fluorescence, Fluorescent Indicators and Probes,
assay   ≥75% (HPLC)
solubility   DMF: soluble
  methanol: soluble
fluorescence   λex 490 nm; λem 514 nm (pH 8.2)
  λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0
suitability   suitable for fluorescence
storage temp.   −20°C
SMILES string   Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)C(=O)NCCCCCC(=O)ON6C(=O)CCC6=O)c1.Oc7ccc8c(Oc9cc(O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)NCCCCCC(=O)ON%12C(=O)CCC%12=O)c7
InChI   1S/2C31H26N2O10/c34-18-6-9-22-24(15-18)41-25-16-19(35)7-10-23(25)31(22)21-8-5-17(14-20(21)30(40)42-31)29(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37;34-18-6-9-21-24(15-18)41-25-16-19(35)7-10-22(25)31(21)23-14-17(5-8-20(23)30(40)42-31)29(39)32-13-3-1-2-4-28(38)43-33-26(36)11-12-27(33)37/h2*5-10,14-16,34-35H,1-4,11-13H2,(H,32,39)
InChI key   WAGYELYAQOYZFU-UHFFFAOYSA-N

Description

Application

6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester (FHS) is used as a reagent to conjugate the fluorophore 6-[Fluorescein-5(6)-carboxamido]hexanoic acid to molecules.6-[Fluorescein-5(6)-carboxamido]hexanoic acid is used as a fluorescein-based fluorescent label for proteins that can be analyzed in applications such as quenching experiments. The hexanoic spacer renders the reactive group more accessible to nucleophiles on biopolymers.

Reagent for introducing the fluorescein label; the spacer renders the reactive group more accessible to nucleophiles on biopolymers

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

MSMLS Online Plate Map

Metabolite descriptors included with the software download upon purchase of the product include: Name, Parent CID, molecular formula, molecular weight, CAS, ChEBI, HMDB ID, PubChem Compound and Subst...
Keywords: Mass spectrometry, Metabolites

Peer-Reviewed Papers
15

References

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