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47036 Sigma-Aldrich

Saponin

for molecular biology, used as non-ionic surfactant

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Core Bioreagents, Detergents,
grade   for molecular biology
mol wt   1000-2000 g/mol
composition   Sapogenin, 8-25%
application(s)   HPLC: suitable
  protein quantification: suitable
CMC   0.001-0.01%
density   1.015-1.020 g/mL at 20 °C (5% in H2O)(lit.)
foreign activity   DNase, RNase, Protease, Phosphatase, none detected

Description

Application

Glycoside widely distributed in plants. Nonionic sufactant that forms oil-in-water emulsions and acts as protective colloid. Used for the direct determination of metals in milk by flame atomic-absorption spectrophotometry. Also used in permeabilization of cell membranes and separation of low molecular weight contaminants, and as an adjuvant in vaccine development.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
VQ1400000

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Protocols & Articles

Articles

Choosing the optimal vaccine adjuvant

The word "vaccination" was first coined by English scientist Edward Jenner in 1796, although early forms of vaccination have been documented as far back as the 17th century.1 Vaccination is used to g...
BioFiles Vol. 5, No. 3
Keywords: Cancer, Clinical, Diseases, Inflammation, Veterinary

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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