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47608 Sigma-Aldrich

Formaldehyde solution

for molecular biology, BioReagent, ≥36.0% in H2O (T)

Synonym: Formalin

  • CAS Number 50-00-0

  • Linear Formula HCHO

  • Molecular Weight 30.03

  •  Beilstein/REAXYS Number 1209228

  •  MDL number MFCD00003274

  •  PubChem Substance ID 329757328

  •  NACRES NA.52

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Properties

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Quality Level   100
grade   for molecular biology
vapor density   1.03 (vs air)
vapor pressure   52 mmHg ( 37 °C)
product line   BioReagent
assay   36.0-38.0% (aldehyde method)
form   liquid
autoignition temp.   572 °F
contains   ~10% methanol as stabilizer
concentration   ≥36.0% in H2O (T)
impurities   ≤0.03% free acid (as HCOOH)
ign. residue   ≤0.05% (as SO4)
pH   2.8-4.0
density   1.09 g/mL at 20 °C
  1.09 g/mL at 25 °C (lit.)
anion traces   chloride (Cl-): ≤10 mg/kg
  sulfate (SO42-): ≤20 mg/kg
cation traces   Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤20 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Na: ≤100 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Zn: ≤1 mg/mL
λ   2 M in H2O
UV absorption   λ: 260 nm Amax: 0.01
  λ: 280 nm Amax: 0.01
suitability   in accordance for electrophoresis of RNA in agarose gels
storage temp.   room temp
SMILES string   [H]C([H])=O
InChI   1S/CH2O/c1-2/h1H2
InChI key   WSFSSNUMVMOOMR-UHFFFAOYSA-N

Description

General description

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.

Application

Suitable for
• denaturation and separation of RNA molecules
• anti-bacterial treatment
• fixation of cells

Caution

may contain precipitate (paraformaldehyde)

Principle

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

Safety & Documentation

Safety Information

Signal word 
Danger
Target organs 
Respiratory system
RIDADR 
UN 2209 8 / PGIII
WGK Germany 
WGK 3
Flash Point(F) 
133.0 °F - closed cup
Flash Point(C) 
56.11 °C - closed cup
Protocols & Articles

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular materia...
Keywords: Adhesion, Cancer, Cell attachment, Cell culture, Cryopreservation, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Vitamins

Formaldehyde Solution for molecular biology

This product is a solution of approximately 37% by weight of formaldehyde gas in water. The product contains 10% methanol as a stabilizer to prevent polymerization. But it can anyway contain precipit...
Keywords: Electrophoresis, Molecular biology, Polymerization reactions

Peer-Reviewed Papers
15

References

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