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47614 Sigma-Aldrich

Fluram

BioReagent, suitable for fluorescence, ≥99.0% (UV)

Synonym: 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione, Fluorescamine

  • CAS Number 38183-12-9

  • Empirical Formula (Hill Notation) C17H10O4

  • Molecular Weight 278.26

  •  Beilstein/REAXYS Number 921143

  •  EC Number 253-814-5

  •  MDL number MFCD00005928

  •  PubChem Substance ID 329757329

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Properties

Related Categories Alphabetical, Amino Acid Analysis, Amino Acids, Analytical Reagents, Analytical/Chromatography,
Quality Level   100
product line   BioReagent
assay   ≥99.0% (UV)
mp   153-157 °C (lit.)
  153-157 °C
solubility   acetonitrile: soluble
  ethanol: soluble
fluorescence   λex 234 nm
  λex 390 nm; λem 480 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)
suitability   suitable for fluorescence
SMILES string   O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14
InChI   1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
InChI key   ZFKJVJIDPQDDFY-UHFFFAOYSA-N

Description

Application

Fluram (fluorescamine) is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides and other molecules to form stable, highly fluorescent compounds. Fluorescamine is useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes and as a pre-column derivatization reagent. It effectively blocks newly generated amino termini in protein sequence analyses.

Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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