47671 Sigma-Aldrich


BioUltra, for molecular biology, ≥99.5% (T)

Synonym: Amide C1, Formic amide

  • CAS Number 75-12-7

  • Linear Formula HCONH2

  • Molecular Weight 45.04

  •  Beilstein/REAXYS Number 505995

  •  EC Number 200-842-0

  •  MDL number MFCD00007941

  •  PubChem Substance ID 57651075

  •  NACRES NA.25



Related Categories Acids and Bases, Alphabetical, BioUltra, Biochemicals and Reagents, DNA & RNA Purification,
Quality Level   200
grade   for molecular biology
vapor density   1.55 (vs air)
vapor pressure   0.08 mmHg ( 20 °C)
  30 mmHg ( 129 °C)
product line   BioUltra
assay   ≥99.5% (T)
autoignition temp.   932 °F
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.1%
refractive index   n20/D 1.447 (lit.)
  n20/D 1.447
pH   7-9 (25 °C, 1 M in H2O)
bp   210 °C (lit.)
mp   2-3 °C (lit.)
solubility   H2O: 10 M at 20 °C, clear, colorless
density   1.134 g/mL at 25 °C (lit.)
anion traces   chloride (Cl-): ≤1 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Ag: ≤5 mg/kg
  Al: ≤1 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤1 mg/kg
  Bi: ≤1 mg/kg
  Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤20 mg/kg
  Li: ≤1 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Mo: ≤1 mg/kg
  NH4+: ≤1000 mg/kg
  Na: ≤20 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Sr: ≤1 mg/kg
  Tl: ≤5 mg/kg
  Zn: ≤1 mg/kg
SMILES string   NC=O
λ   10 M in H2O
UV absorption   λ: 260 nm Amax: ≤0.4
  λ: 280 nm Amax: ≤0.1
InChI   1S/CH3NO/c2-1-3/h1H,(H2,2,3)



Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other Notes

For the preparation of formamide denaturing polyacrylamide gels; In situ hybridization on human chromosomes

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
Target organs 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
347.0 °F
Flash Point(C) 
175 °C

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the best way to deionize Formamide, Product 47671?
Mixed Bed Resin (e.g., Sigma Product No. M8157 or M8032) can be used to deionize formamide.
Are aqueous solutions ofFormamide, Product 47671, stable?
In aqueous solution, formamide hydrolyzes to form ammonia and formic acid (i.e., ammonium formate).
What is the molarity of the neat liquid Formamide, Product 47671?
Based on the density of 1.13 and the molecular weight of 45.04, the molarity of this neat liquid is 25.09 M. The pH of a 0.5 M aqueous solution is 7.1, according to The Merck Index, 12th edition.
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Protocols & Articles


GC Analysis of Class 2 Residual Solvents on OVI-G43

From our library of Articles, Sigma-Aldrich presents GC Analysis of Class 2 Residual Solvents on OVI-G43
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