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49752 Sigma-Aldrich

2-Phospho-L-ascorbic acid trisodium salt

≥95.0% (HPLC)

Synonym: L-Ascorbic acid 2-phosphate trisodium salt, Sodium L-ascorbyl-2-phosphate

  • CAS Number 66170-10-3

  • Empirical Formula (Hill Notation) C6H6Na3O9P · xH2O

  • Molecular Weight 322.05 (anhydrous basis)

  •  MDL number MFCD03095479

  •  NACRES NA.79

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Properties

Related Categories Biochemicals and Reagents, Vitamin C, Vitamins More...
biological source   synthetic
assay   ≥95.0% (HPLC)
form   powder
application(s)   cell culture | mammalian: suitable
impurities   ≤7.5% water (Karl Fischer)
color   white to off-white
pH   9.0-9.5 (25 °C, 30 g/L in H2O)
mp   260 °C
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤10 mg/kg
  As: ≤2 mg/kg
  Ba: ≤5 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤10 mg/kg
  Mg: ≤10 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
storage temp.   room temp

Description

Application

2-Phospho-L-ascorbic acid trisodium salt has been used as medium supplement in Dulbecco′s Modified Eagle Medium (DMEM) for adipose-derived stem cells, human mesenchymal stem cells and osteoblast cells.

Biochem/physiol Actions

L-Ascorbic acid 2-phosphate (AA2P) is used in biocatalytic dephosphorylation for electric power generation and electrochemical detection assays. L-Ascorbic acid 2-phosphate may be used in cell differentiation and tissue engineering applications. Asc-2P is used in studies of gene repression, such as the repression of dihydrotestosterone-induced dickkopf-1 expression.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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