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5008 Sigma-Aldrich

VitroCol®

protein from human, suitable for cell culture

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Properties

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biological source   protein from human
sterility   sterile-filtered
form   lyophilized
packaging   pkg of 15 mg
mfr. no.   Advanced BioMatrix
impurities   <1 EU/mL Endotoxin level
suitability   suitable for cell culture
shipped in   wet ice
storage temp.   −20°C

Description

General description

VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblasts to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.

VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains high monomer content as measured by gel permeation chromatography.

VitroCol® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. It is not recommend for the formation of a solid gel. VitroCol® is supplied as a sterile, lyophilized powder.

Preparation Note

This product is supplied as a lyophilized powder with 15 mg of human collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Legal Information

VitroCol is a registered trademark of Advanced BioMatrix, Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide
Protocols & Articles

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