5090 Sigma-Aldrich

CDH18 human

recombinant, expressed in E. coli, 0.5 mg protein/mL



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biological source   human
recombinant   expressed in E. coli
description   0.1 mg of recombinant human CDH18 in 20 mM Tris-HCl buffer, containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol.
sterility   Filtered sterilized solution
assay   ≥90% (SDS-PAGE)
form   liquid
packaging   pkg of 100 μg
concentration   0.5 mg protein/mL
accession no.   NP_004925
UniProt accession no.   Q13634
storage temp.   −20°C
Gene Information   human ... CDH18(1016)



Coating a plate well (6 well plate) with this recombinant CDH18 protein in neuronal cell specific medium at 1-10 μg/well allows for 1) human neuronal cell / receptor interaction studies or 2) use as a culture matrix protein for human neuronal axon connection studies in vitro.

Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice.
1. Thaw CDH18 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so the volume added covers the surface evenly (1-10 μg/well, 6 well plate).
2. Add appropriate amount of diluted material to culture surface.
3. Incubate at room temperature for approximately 1.5 hours.
4. Aspirate remaining material.
5. Rinse plates carefully with water and avoid scratching bottom surface of plates.
6. Plates are ready for use. They may also be stored at 2-8 °C damp or air dried if sterility is maintained.



Preparation Note

The full-length extracellular domain of the human CDH18 gene (54-608 aa) was constructed with 29 N-terminal T7/HIS-tag and expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

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NONH for all modes of transport
WGK Germany 


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