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50981 Sigma-Aldrich

Guanidine thiocyanate

BioReagent, for molecular biology, ≥99%

Synonym: Guanidinium rhodanide, Guanidinium thiocyanate

  • CAS Number 593-84-0

  • Linear Formula NH2C(=NH)NH2 · HSCN

  • Molecular Weight 118.16

  •  Beilstein/REAXYS Number 3563461

  •  EC Number 209-812-1

  •  MDL number MFCD00013027

  •  PubChem Substance ID 57651291

  •  NACRES NA.52

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Properties

Related Categories Biochemicals and Reagents, Chaotropic Agents, Denaturation More...
biological source   synthetic
grade   for molecular biology
product line   BioReagent
assay   ≥99%
form   powder
application(s)   RNA extraction: suitable
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
color   colorless to white,

mp   115-122 °C
  120-122 °C (lit.)
solubility   H2O: 4 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤5000 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤5000 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
storage temp.   room temp
SMILES string   SC#N.NC(N)=N
InChI   1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
InChI key   ZJYYHGLJYGJLLN-UHFFFAOYSA-N

Description

Application

Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.

Chaotropic agent and strong denaturant; solubilizes cells.

Packaging

100, 500 g in glass bottle

Biochem/physiol Actions

Guanidine thiocyanate (GTC) plays a role in protein denaturation. It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP). It can be used along with phenol and chloroform for extraction of RNA.

Principle

Guanidine thiocyanate interferes with the hydrogen bonds and intramolecular interactions of macromolecules. It solubilizes the proteins resulting in disintegration of cellular structures.

Safety & Documentation

Safety Information

Signal word 
Danger-Danger
Supplemental Hazard Statements 
EUH032EUH032
Personal Protective Equipment 
RIDADR 
UN 1759 8 / PGIII
WGK Germany 
WGK 2
RTECS 
XL1225000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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