5100 Sigma-Aldrich

CD164 human

recombinant, expressed in E. coli, 0.5 mg protein/mL



Related Categories Attachment Factors, CD160 to CD340, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
biological source   human
recombinant   expressed in E. coli
description   0.05 mg of recombinant human CD164 in 20 mM Tris-HCl buffer, containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol.
sterility   Filtered sterilized solution
assay   ≥90% (SDS-PAGE)
form   liquid
packaging   pkg of 50 μg
concentration   0.5 mg protein/mL
accession no.   NP_006007
UniProt accession no.   Q04900
storage temp.   −20°C
Gene Information   human ... CD164(8763)



Coating a plate well (6 well plate) with this recombinant CD164 matrix protein in HSC cell specific medium at 1-10 μg/well allows for human HSC / receptor interaction studies in vitro.

Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice.
1. Thaw CD164 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so the volume added covers the surface evenly (1-10 μg/well, 6 well plate).
Note: Use 1 ml PBS per well in a 6-well plate.
2. Add 1 - 10 μg protein to each well and incubate at 2 to 10 °C overnight.
3. After incubation, aspirate remaining material.
4. Plates are ready for use. They may also be stored at 2-8 °C damp or air dried if sterility is maintained



Preparation Note

The full-length extracellular domain of the human CD164 cDNA (24 - 162 aa) was constructed with 29 N-terminal T7/HIS-tag and expressed in E. coli as inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified as soluble protein.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


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