EMAIL THIS PAGE TO A FRIEND
51456 Sigma-Aldrich

Urea

BioUltra, for molecular biology, ≥99% (T)

Synonym: Carbamide, Carbonyldiamide

  • CAS Number 57-13-6

  • Linear Formula NH2CONH2

  • Molecular Weight 60.06

  •  Beilstein/REAXYS Number 635724

  •  EC Number 200-315-5

  •  MDL number MFCD00008022

  •  PubChem Substance ID 57651307

  •  NACRES NA.25

Purchase

Properties

Related Categories Alphabetical, BioUltra, Biochemicals and Reagents, Chaotropic Agents, DNA & RNA Purification,
Quality Level   200
grade   for molecular biology
product line   BioUltra
assay   ≥99% (T)
form   powder or crystals
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
  ≤0.1% biuret
  <0.005% formaldehyde (HCHO)
ign. residue   ≤0.01% (as SO4)
loss   ≤0.1% loss on drying, 20 °C (HV)
pH   8.0-10.0 (20 °C, 8 M in H2O)
mp   132-135 °C (lit.)
solubility   H2O: 8 M at 20 °C, clear, colorless
density   1.335 g/mL at 25 °C (lit.)
anion traces   chloride (Cl-): ≤5 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
SMILES string   NC(N)=O
λ   8 M in H2O
UV absorption   λ: 260 nm Amax: ≤0.03
  λ: 280 nm Amax: ≤0.02
InChI   1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Other Notes

Denaturing reagent; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
YR6250000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?