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5190 Sigma-Aldrich

CytoSoft®, Discovery Kit

Multiple Elastic Moduli, (0.2, 0.5, 2, 8, 16, 32, 64 kPa)

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Properties

Related Categories Cell Culture Supplies, Culture Multiwell Plates, Labware More...
sterility   irradiated
form   solid
packaging   pack of 7 plates
application(s)   cell culture | mammalian: suitable
capacity   2.0-3.0 mL
size   6 wells
surface area   9.5 cm2
matrix active group   Functionalized silicone surface treatment
solubility   water: insoluble

Description

General description

The rigidity of the substrate to which cells adhere can have a profound effect on cell morphology and gene expression. CytoSoft® products provide a tool to culture cells on substrates with various rigidities covering a broad physiological range. On the bottom of each well, there is a thin layer of specially formulated biocompatible silicone, whose elastic modulus (rigidity) is carefully measured. The surfaces of the gels in CytoSoft® products are functionalized to form covalent bonds with amines on proteins. This chemical functionalization is stable and the reaction does not require a catalyst, facilitating the coating of the gel surfaces with matrix proteins and plating cells.

The silicone substrates of CytoSoft® products are optically clear and have a low auto-florescence. The layer of silicone in each dish is firmly bonded to the bottom of the dish. Unlike hydrogels (such as polyacrylamide gels), silicone gels are not susceptible to hydrolysis, do not dry nor swell, are resilient and resistant to tearing or cracking, and their elastic moduli (rigidities) remain nearly unchanged during extended storage periods.

CytoSoft® products accommodate the harvesting of cells using enzymes such as trypsin and collagenase. There is no biochemical breakdown of the substrate during or after enzyme treatment, and there are no residuals of the substrate in the sample retrieved from a CytoSoft® plate.

This CytoSoft® product, Catalog Number 5190-7EA, has various elastic moduli of approximately 0.2, 0.5, 2, 8, 16, 32 and 64 kPa in 7 individual 6-well plates. The thickness of the silicone gel is uniform with a ~0.5 mm thick layer of silicone in each dish that is fully compatible with mammalian cell cultures. The plates are packaged individually, sterilized using irradiation and provided with 7 dishes per package.

Legal Information

CytoSoft is a registered trademark of Advanced BioMatrix, Inc.

Cytodex is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

CytoSoft® Elastic Modulus Plates: An Innovative Tool to Analyze the Effect of Matrix Stiffness/Rigidity on Regulating Cellular Behavior

Mechanotransduction refers to the processes through which cells sense and respond to mechanical stimuli by converting them to biochemical signals that elicit specific cellular responses. Focal adhesi...
Keywords: Adhesion, Cancer, Catalysis, Gene expression, Ligands, Reductions

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

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