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52412 Sigma-Aldrich

R-Phycoerythrin

BioReagent, passes test for gel electrophoresis

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains, Luminescent Compounds/Detection, Phycobili Proteins,
Quality Level   100
product line   BioReagent
concentration   ≥10.0 mg/mL (UV)
fluorescence   λex 542 nm; λem 575 nm in 0.1 M phosphate pH 7.2
suitability   passes test for gel electrophoresis
storage temp.   2-8°C

Description

General description

The product is suspended in 150 mM sodium phosphate, 60% ammonium sulfate, 1 mM EDTA, 1 mM sodium azide, pH 7.0 and must be dialyzed against conjugation buffer or PBS before conjugation.

Application

R-Phycoerythrin (R-PE), is useful in the laboratory as a fluorescence-based indicator for the presence of cyanobacteria and for a variety of immunofluorescence applications. R-PE is a useful fluoroprobe in various electrophoretic procedures.

Analysis Note

A566/A280 >4.6, A620/A566 <0.03, A566/A498 <1.5

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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