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  • 52953 - Anti-Mouse IgG−Abberior® CAGE 500 antibody produced in goat

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52953 Sigma-Aldrich

Anti-Mouse IgG−Abberior® CAGE 500 antibody produced in goat

for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

Synonym: Abberior® CAGE 500-Anti-Mouse-IgG antibody produced in goat

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Properties

Related Categories Alphabetical Index, Antibodies, MO-MR, Primary Antibodies
antibody product type   primary antibodies
availability   available only in USA and Canada
fluorescence   λex 510 nm; λem 530 nm±10 nm in PBS
suitability   corresponds for SDS-PAGE
storage temp.   −20°C

Description

Analysis Note

May contain significant amounts of BSA as stabilizer

unconjugated dye≤5% of total fluorescence

General description

Abberior CAGE 500 is a masked dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye with spectral properties essentially identical to Abberior STAR 512. The dye performs e.g. very well in the Nikon N-Storm, particularly as a 2-color partner with CAGE552 or FLIP565.

Absorption Maximum, λmax: 230, 299, 340 nm (non-activated, PBS, pH 7.4)
511 nm (photoactivated, PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 50,000 (photoactivated, PBS, pH 7.4))
Fluorescence Maximum, λfl: 525 nm (PBS, pH 7.4)
Photoactivation Wavelength: 360 - 440 nm
Recommended STED Wavelength, λSTED: 595 − 615 nm
Fluorescence Quantum Yield, η: 0.85 (after photoactivation, PBS, pH 7.4)
Fluorescence Lifetime, τ: 4.1 (PBS, pH 7)

Other Notes

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Legal Information

abberior is a registered trademark of Abberior GmbH

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

Related Products

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Product #

Description

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44254 Abberior® CAGE 500, NHS ester, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
92546 Abberior® CAGE 500, maleimide, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
14395 Abberior® CAGE 500, phalloidin, for STED application
41155 Anti-Rabbit IgG−Abberior® CAGE 500 antibody produced in goat, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

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