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  • 53399 - Anti-Rabbit IgG−Abberior® STAR 635P antibody produced in goat

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53399 Sigma-Aldrich

Anti-Rabbit IgG−Abberior® STAR 635P antibody produced in goat

for STED application

Synonym: Abberior® STAR 635P-Anti-Rabbit IgG antibody produced in goat

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RAB-RAD
clone   polyclonal
biological source   goat
species reactivity   rabbit
form   buffered aqueous solution
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   secondary antibodies
concentration   ~1 mg/mL
fluorescence   λex 638 nm; λem 651 nm in PBS, pH 7.4

Description

General description

Abberior STAR 635P was the first member of an entirely new class of fluorescent dyes introduced exclusively by Abberior: the class of phosphorylated fluorescent dye. The dye excels in its photophysical parameters: it is very bright, very stable and it enables basically background free imaging. The dye works exceptionally well with Abberior Instruments STEDYCON und Expert line microscopes and Leica STED microscopes. Abberior STAR 635P can substitute Atto 647N, Alexa Fluor® 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Abberior STAR 635P is the ideal partner for Abberior STAR 580 to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.

Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 120 000 (H2O), 130 000 (MeOH)
Correction Factor, CF260 = ε260max: 0,21 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,40 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 651 (PBS pH 7.4), 651 (H2O), 651 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,3 (PBS pH 7.4)

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Atto is a trademark of Atto-Tec GmbH

Cy is a registered trademark of GE Healthcare

abberior is a registered trademark of Abberior GmbH

Suitability

Designed and tested for fluorescent super-resolution microscopy

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Other Notes

Find more infomation here Abberior®

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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Product #

Description

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04683 Abberior® STAR 635P, streptavidin

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