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  • 540-05A - Human Lung Microvascular Endothelial Cells: HLMVEC, adult

540-05A Sigma-Aldrich

Human Lung Microvascular Endothelial Cells: HLMVEC, adult

  •  NACRES NA.81



Related Categories Cell Culture, Endothelial Cells, Primary Cell Culture, Primary Cells
Quality Level   100
biological source   human lung (normal)
packaging   pkg of 500,000 cells
growth mode   Adherent
karyotype   2n = 46
morphology   Endothelial
application(s)   cell culture | mammalian: suitable
relevant disease(s)   metastasis
shipped in   dry ice
storage temp.   −196°C


General description

The pulmonary vasculature is of great physiological/pathological significance. It is now recognized to be a tissue composed of metabolically active, functionally responsive cells, that interact with circulating substrates and regulate the composition of systemic arterial blood, affect target organ functions, and contribute to thrombosis, hemostasis and immune reactions, as well as tumor metastasis. Human Lung Microvascular Endothelial Cells (HLMVEC) provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro.

HLMVEC have been used to elucidate the therapeutic effects of Angiotensin I-converting enzyme (ACE) inhibitors, and the results revealed that they provide an additional benefit to patients by activating bradykinin B1 receptor leading to prolonged nitric oxide (NO) production in endothelial cells and inhibition of PKCε (Ignjatovic, 2004; Stanisavljevic, 2006).

Cell Line Origin



lung physiology, metabolism, regulate blood composition, hemostasis, immune reactions, therapeutic effects, leukocyte migration,


Cell Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

• 3rd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 15 doublings

Subculture Routine

Please refer to the HLMVEC Culture Protocol.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


Protocol: Human Lung Microvascular Endothelial Cells Culture

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