• USA Home
  • 56500 - GenElute Agarose Spin Columns

56500 Sigma-Aldrich

GenElute Agarose Spin Columns

sufficient for 70 purifications

Synonym: DNA purification from agarose gel, Gen Elute

  •  NACRES NA.52



General description

GenElute Agarose Spin Columns allow for the purification of linear DNA from agarose gels. The typical recovery is 40 to 45% for 100 bp to 10 kb DNA fragments. The recovery decreases as the fragment size increases.


The purified DNA can be used in most downstream molecular biology applications including ligation, PCR, restriction digest, cloning, labeling and hybridization.

Features and Benefits

• One-step, 10 minute protocol
• No melting or digestion of agarose required
• Simpler and quicker than bind and elute or electroelution methods
• No ethanol precipitation
• Typical recovery of 40 to 45% for DNA fragments from 100 bp to 10 kb (Figs. 1 and 2)

Other Notes

GenElute Agarose Spin Columns isolate DNA by centrifugation/filtration.

For additional information, please see www.sigma-aldrich.com/post-reaction.


GenElute Agarose Spin Columns are designed for the rapid purification of linear DNA (100 bp to 10 kb) from agarose gels. The DNA band is excised from an agarose gel and loaded into the spin column. Embedded within the base of the column are a series of membranes and filters that hold agarose and impurities back while allowing DNA to selectively pass through the column into a collection tube during a 10 minute centrifugation. GenElute Agarose Spin Columns eliminate the need for silica-based resins, DEAE or toxic organic solvents such as phenol and chloroform. There is no melting, electroelution or enzymatic digestion of the agarose gel.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles


Nancy-520 for DNA Detection and Quantitation

Nancy-520 is a fluorescent stain for double stranded DNA (dsDNA) on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is...
Monika Baeumle
BioFiles 2009, 4.1, 4.
Keywords: Catalytic combustion detector, Electrophoresis

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?