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56741 Supelco

IGEPAL® CA-630

Synonym: Nonidet P 40, Nonylphenyl-polyethylenglycol, Octylphenoxy poly(ethyleneoxy)ethanol, branched

  • CAS Number 9002-93-1

  • Linear Formula (C2H4O)nC14H22O

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Properties

Related Categories Acrylamides, Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Detergents,
description   non-ionic
mol wt   ~603.0 g/mol
application(s)   thin layer chromatography (TLC): suitable
refractive index   n20/D 1.492
CMC   0.08 mM (20-25°C)
transition temp   cloud point 63-67  °C
density   1.06 g/mL at 20 °C
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤100 mg/kg
cation traces   Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤1000 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤1000 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg
HLB   13

Description

General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL® CA-630 has been used:
• In phosducin co-precipitation assay for pull down of Epitope-Tagged HAGγ1 and HAGγ1C71S
• As a component of lysis buffer for cell culture and sample preparation for MAPK (mitogen-activated protein kinases) analysis, Immunoblotting
• In the extraction of cells in Oil Red O staining procedure to visualise lipid formation in cells during adipogenesis
• As a component of lysis buffer containing Tris-HCl, NaCl and EDTA for immunoblotting experiment

Other Notes

Nonionic, non-denaturing detergent

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Caution

May become turbid on storage; a clear product can be obtained on heating to 40°C.

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Legal Information

IGEPAL is a registered trademark of Rhodia Operations

Nonidet is a trademark of Shell Chemical Co.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
2
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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