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56748 Sigma-Aldrich

Imidazole

BioUltra, for molecular biology, ≥99.5% (GC)

Synonym: 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline

  • CAS Number 288-32-4

  • Empirical Formula (Hill Notation) C3H4N2

  • Molecular Weight 68.08

  •  Beilstein/REAXYS Number 103853

  •  EC Number 206-019-2

  •  MDL number MFCD00005183

  •  eCl@ss 39161001

  •  PubChem Substance ID 57651596

  •  NACRES NA.25

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Properties

Related Categories Alphabetical, BioUltra, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers,
grade   for molecular biology
vapor pressure   <1 mmHg ( 20 °C)
product line   BioUltra
assay   ≥99.5% (GC)
form   flakes
  powder or crystals
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
  ≤0.2% water
ign. residue (900 °C)   ≤0.05%
pH   9.5-11.0 (25 °C, 0.1 M in H2O)
pKa (25 °C)   6.95
bp   256 °C (lit.)
mp   88-91 °C (lit.)
solubility   H2O: 0.1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
SMILES string   c1c[nH]cn1
λ   0.1 M in H2O
UV absorption   λ: 260 nm Amax: 0.10
  λ: 280 nm Amax: 0.10
InChI   1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
InChI key   RAXXELZNTBOGNW-UHFFFAOYSA-N

Description

Application

Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.

Excellent for buffers in the range of pH 6.2-7.8

Other Notes

Review: As RNase model

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3263 8 / PGIII
WGK Germany 
WGK 2
RTECS 
NI3325000
Flash Point(F) 
293.0 °F - closed cup
Flash Point(C) 
145 °C - closed cup
Protocols & Articles

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Peer-Reviewed Papers
15

References

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