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57000 Sigma-Aldrich

Indigo carmine

for microscopy (Bact., Hist.), indicator (pH 11.5-14.0)

Synonym: Acid Blue 74, Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine

  • CAS Number 860-22-0

  • Empirical Formula (Hill Notation) C16H8N2Na2O8S2

  • Molecular Weight 466.35

  •  Colour Index Number 73015

  •  Beilstein/REAXYS Number 4103904

  •  EC Number 212-728-8

  •  MDL number MFCD00005723

  •  PubChem Substance ID 57651616

  •  NACRES NA.47

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Properties

Related Categories Cell Biology, G-H-I, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
Quality Level   100
grade   for microscopy (Bact., Hist.)
  indicator (pH 11.5-14.0)
form   powder
application(s)   hematology: suitable
  histology: suitable
loss   ≤5% loss on drying
pH   3.87 (30 °C, 7.14 g/L)
mp   >340 °C
solubility   methanol: 0.01 g/10 mL, blue to very deep blue
density   0.71 g/cm3 at 29 °C
εmax   ≥350 at 603-613 nm
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O
InChI   1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;
InChI key   KHLVKKOJDHCJMG-QDBORUFSSA-L

Description

Application

Indigo carmine has been used in agarose plates to study behavioral feeding assays in Drosophila larvae. It has also been used for macroscopic assessment of intestinal adenomatous polyps isolated from mice.

Biochem/physiol Actions

Indigo carmine is a contrast dye which helps in highlighting mucosal changes. It accumulates in crevices, cysts and foveolae and stains the tissue blue. The abnormal tissue remains translucent pink.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
DU3000000

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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