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59205C SAFC

HEPES Buffer Solution (1M)

Synonym: HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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Properties

description   for research or for further manufacturing use
form   liquid
useful pH range   6.8 - 8.2
storage temp.   2-8°C
SMILES string   OCCN1CCN(CCS(=O)(O)=O)CC1
InChI   1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key   JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

Application

HEPES is used in many media because it has more buffering capacity in the physiologic pH range of 7.2 - 7.4 at 37 C than sodium bicarbonate. It is commonly added at concentrations between 10 mM and 25 mM. Higher levels cause cytotoxicity. Because bicarbonate is nutritionally necessary for most cells, HEPES should be added in addition to, not in place of, sodium bicarbonate.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Peer-Reviewed Papers
15

References

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