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59429C SAFC

Trypsin (1X)

sterile; γ-irradiated, 0.25% porcine trypsin (1:250) in HBSS, w/0.1% EDTA-Na2, w/o Ca++ and Mg++

Synonym: Trypsin-EDTA solution

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Properties

Related Categories Cell Biology, Dissociation, General Stem Cell Biology, Stem Cell Biology, Stem Cell Isolation More...
Quality Level   500
description   for research or for further manufacturing use
sterility   sterile; γ-irradiated
form   liquid
application(s)   cell culture | mammalian: suitable
components   phenol red: 10.620 mg/L
NaHCO3: 350.000 mg/L
shipped in   dry ice
storage temp.   −20°C

Description

Application

Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. They are generally composed of variable concentrations of natural porcine pancreas-derived trypsin and EDTA. Trypsin 1X solutions can range from 0.025% to 0.5%. The reasons for the range of concentrations are differences in required trypsin activity or potency; differences in incubation times; and cell line susceptibility. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.

Preparation Note

SAFC Biosciences has validated a process using γ radiation to significantly reduce the risks associated with adventitious agents such as Porcine Parvovirus (PPV), PorcineRespiratory and Reproductive Syndrome (PRRS) and Mycoplasma hyorhinis while maintaining product performance. The use of γ irradiated trypsin requires no change to the end user’s methods or procedures while giving additional assurance against microbial contaminants associated with animal-derived products. All trypsin is obtained from the United States or other countries deemed free of Bovine Spongiform Encephalopathy (BSE).

This product does contain phenol red. Due to shipment on dry ice, there could be significant carbon dioxide buildup in the package. This CO2 may enter the solution and lower the pH slightly, giving an orange rather than pinkish color. The orange solution will still be suitable for use, or the pH can be adjusted with sodium hydroxide. Incubating cells with too high a trypsin concentration for a long period can damage cell membranes and kill the cells. Solubilizing trypsin or diluting it from a concentrated solution should be done with a buffered salt solution containing no Ca2+ or Mg2+.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

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Description

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T4299 Trypsin-EDTA solution, 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 500 BAEE units porcine trypsin and 180 μg EDTA • 4Na per ml in Dulbecco′s PBS without calcium and magnesium
T4049 Trypsin-EDTA solution, 0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
T3924 Trypsin-EDTA solution, 1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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