• USA Home
  • 602-05A - Hair Follicle Dermal Papilla Cells: HFDPC, adult

EMAIL THIS PAGE TO A FRIEND
602-05A Sigma-Aldrich

Hair Follicle Dermal Papilla Cells: HFDPC, adult

  •  NACRES NA.81

Purchase

Properties

Related Categories Cell Culture, Hair Cell Media, Hair Cells, Media, Primary Cell Culture,
biological source   human scalp (hair follicle papilla)
packaging   pkg of 500,000 cells
growth mode   Adherent
morphology   mesenchymal
application(s)   cell culture | mammalian: suitable
relevant disease(s)   Alzheimer′s disease
shipped in   dry ice
storage temp.   −196°C

Description

General description

Human Hair Follicle Dermal Papilla Cells (HFDPC) are mesenchymal cells isolated from the hair papilla of normal human scalp hair follicles. Hair papilla, in the adult hair follicle, plays a crucial role in the dermal-epidermal interactions that control hair production and events of the hair growth cycle. HFDPC can therefore be used for studying cellular mechanisms organizing the hair follicle, and for the development and evaluation of hair growth products.

HFDPC have been used to demonstrate that IL-1α affects dermal papilla activity by decreasing androgen receptor expression and stimulating secretion of KFG, VEGF, IL-8 and GM-CSF (Boivin, 2006). They were also used to investigate how gene expression and cell proliferation are affected by testosterone analogues (Reiter, 2008), potential hair growth enhancing agents (Sasajima, 2008) and microbial metabolites (Takigawa, 2012). Using HFDPC, along with Human Epidermal Keratinocytes and Human Bronchial Epithelial Cells, scientists also studied mechanisms of cellular senescence, and develop ed methodology to extend cellular life span and immortalize the cells by inhibiting p16INK4a and introducing hTERT (Haga, 2007). Finally, HFDPC have been used to discover the link between the androgen-induced alopecia and circadian rhythms, mediated by a “clock gene” transcription factor BMAL1 (Watabe, 2013).

Cell Line Origin

Scalp

Application

dermatalogical interactions, hair production, induction and maintenance of hair growth, hair follicle organization, development and evaluation of hair growth products, effects and secretion of growth factors and cytokines, gene expression, cell proliferation, cell senescence, cell lifespan extension, in vitro screening of androgen blocking agents, reservoir of multi-potent stem cells, skin reconstruction, wound healing, signal transduction pathways, skin composites and substitutes, hair follicle neogenesis

Components

Cell Basal Medium containing 20% FBS & 5% DMSO

Preparation Note

• 2nd passage, >500,000 cells in Cell Basal Medium containing 20% FBS & 5% DMSO
• Can be cultured at least 6 doublings

Subculture Routine

Please refer to the HFDPC Culture Protocol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Pipette Controller from Brand
Protocols & Articles

Protocols

Protocol: Human Follicle Dermal Papilla Cells Culture

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Introduction to Primary Cell Culture [VIDEO]

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cell culture, Drug discovery, Pharmaceutical

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?