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62288 Sigma-Aldrich

Lipase B Candida antarctica, recombinant from Aspergillus oryzae

powder, beige, ~9 U/mg

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Description

General description

Candida antarctica lipase B (CalB) is structurally similar to several other lipases and has a flexible lid. It is made up of 317 amino acids and has a molecular weight of 33 kDa. Lipase B is a member of the alpha/beta hydrolase-fold family.

Application

Lipase B Candida antarctica, recombinant from Aspergillus oryzae has been used:
• as a standard to characterize the enzymatic properties of D5-CalB
• as an efficient biocatalyst to start the reaction to obtain (R)-ester via esterification of racemic secondary alcohol
• to investigate a “green” recycling route for polybutylene succinate (PBS) based on reactive extrusion
• to compare the esterification yield with adsorbed CaLB (aCaLB) and covalently immobilized CaLB (cCaLB)

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Candida antarctica lipase B (CALB) possesses wide substrate specificity, high activity and high enantioselectivity, hence it is considered as a major enzyme in biotechnology. It also has the capability to perform in aqueous and non-aqueous reaction environments. CALB is used in transesterification, kinetic resolution and polymerization reactions.

Lipase B from Candida antarctica has been shown to be an effective catalyst for the synthesis of esters of ethyl D-glucopyranoside from fatty acids larger than octanoic acid. It has also been found to catalyze a wide variety of organic reactions including many different regio- and enantio-selective syntheses.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 8.0 and 40°C (tributyrin, Cat. No. 91010, as substrate)

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TO9776500

Documents

Certificate of Analysis

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What solvent can Lipase B Candida antarctica, recombinant from Aspergillus oryzae, Product 62288, be dissolved in?
Lipase B is expected to be soluble in water. We are able to dissolve the enzyme in water for the enzymatic assay. We also have tested the solubility of Lipase B in 0.05M potassium phosphate buffer solution (pH=7.5) during quality control at a concentration of 10mg/mL. According to the Merck Index, almost all organic solvents decrease lipase activity, with the exception of petroleum ether.
Is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae, lyophilized?
This lipase is a lyophilisate and may contain Gum arabic for adjustment of activity. The amount of Gum arabic in each vial is lot dependent.
How pure is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae?
Purity is not determined for this product. As is the case with many of our enzymes, the product is assayed for enzymatic activity, not for purity.
What is the molecular weight of Lipase B Candida antarctica, recombinant from Aspergillus oryzae, Product 62288?
Unfortunately, molecular weight information is unavailable at this time.
What is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae, soluble in?
Lipase B is expected to be soluble in water. We are able to dissolve the enzyme in water for the enzymatic assay. We also have tested the solubility of Lipase B in 0.05 M potassium phosphate buffer solution (pH=7.5) during quality control at a concentration of 10 mg/mL. According to the Merck Index, almost all organic solvents decrease lipase activity, with the exception of petroleum ether.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Peer-Reviewed Papers
15

References

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