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63689 Sigma-Aldrich

2-Mercaptoethanol

BioUltra, for molecular biology, ≥99.0% (GC)

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

  • CAS Number 60-24-2

  • Linear Formula HSCH2CH2OH

  • Molecular Weight 78.13

  •  Beilstein/REAXYS Number 773648

  •  EC Number 200-464-6

  •  MDL number MFCD00004890

  •  PubChem Substance ID 57651953

  •  NACRES NA.25

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Properties

Related Categories BioUltra, Molecular Biology, Molecular Biology Reagents More...
Quality Level   200
grade   for molecular biology
vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
product line   BioUltra
assay   ≥99.0% (GC)
form   liquid
expl. lim.   18 %
concentration   14.3 M (pure liquid)
application(s)   RNA extraction: suitable
  protein extraction: suitable
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
refractive index   n20/D 1.500 (lit.)
  n20/D 1.501
pH   4.5-6 (20 °C, 500 g/L)
bp   157 °C (lit.)
solubility   H2O: 0.5 M at 20 °C, clear, colorless
density   1.114 g/mL at 25 °C (lit.)
cation traces   Al: ≤0.5 mg/kg
  Ba: ≤0.1 mg/kg
  Bi: ≤0.1 mg/kg
  Ca: ≤0.5 mg/kg
  Cd: ≤0.05 mg/kg
  Co: ≤0.02 mg/kg
  Cr: ≤0.02 mg/kg
  Cu: ≤0.02 mg/kg
  Fe: ≤0.1 mg/kg
  K: ≤0.5 mg/kg
  Li: ≤0.1 mg/kg
  Mg: ≤0.1 mg/kg
  Mn: ≤0.02 mg/kg
  Mo: ≤0.1 mg/kg
  Na: ≤1 mg/kg
  Ni: ≤0.05 mg/kg
  Pb: ≤0.1 mg/kg
  Sr: ≤0.1 mg/kg
  Zn: ≤0.1 mg/kg
SMILES string   OCCS
λ   0.5 M in H2O
UV absorption   λ: 260 nm Amax: 1.5
  λ: 280 nm Amax: 0.3
storage temp.   2-8°C
InChI   1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
InChI key   DGVVWUTYPXICAM-UHFFFAOYSA-N

Description

Application

2-Mercaptoethanol has been used
• in the protein extraction buffer prepared for leaf samples.
• as a supplement in cell culture media.
• in the procedure of RNA extraction.

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds

Safety & Documentation

Safety Information

Signal word 
Danger
RIDADR 
UN 2966 6.1 / PGII
WGK Germany 
WGK 3
RTECS 
KL5600000
Flash Point(F) 
165.2 °F - closed cup
Flash Point(C) 
74 °C - closed cup
Protocols & Articles

Protocols

Hand-casting gels for PAGE and SDS-PAGE using TurboMix™ Bis-Tris Gel Casting Kits

Polyacrylamide gel electrophoresis (PAGE) is a foundational technique used to separate proteins and other macromolecules by their electrophoretic mobility. Polyacrylamide gels are formed through the ...
Keywords: Alkylations, Cell disruption, Deaminations, Degradations, Electrophoresis, Gel electrophoresis, Nucleic acid denaturation, PAGE, Polymerization reactions, Sample preparations, Separation, Titrations

Peer-Reviewed Papers
15

References

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