• USA Home
  • 65497 - TRIS acetate-EDTA Buffer solution

65497 Sigma

TRIS acetate-EDTA Buffer solution

for Northern and Southern blotting, solution



Related Categories Buffers, Molecular Biology, Protein Electrophoresis, Proteomics, SDS-PAGE More...
grade   for molecular biology
product line   BioReagent
form   solution
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
pH   8.3±0.2
density   1.00 g/mL at 20 °C
storage temp.   room temp



The TAE liquid buffer is a 1x solution that contains 0.04M TRIS acetate and 0.001M EDTA in water.


Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. Especially useful for dsDNA separations.

General description

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This concentrate can be easily diluted to 0.5x before use (with molecular biology grade water).

Price and Availability

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Related Products

related product

Product #


Add to Cart

B6429 Casein Blocking Buffer 10x, for Northern and Southern blotting, solution
D0428 1 kb DNA Ladder, for DNA electrophoresis
H7033 PerfectHyb Plus Hybridization Buffer, for Northern and Southern blotting, solution
T9285 Tris-EDTA buffer solution, 100X concentrate for Northern and Southern blotting
S6639 SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution
B6185 Bionic Buffer, 10X Concentrate, for Northern and Southern blotting, solution
GERPN3005 ECL Direct Nucleic Acid, GE Healthcare, RPN3005, pack of 1 ea
SRE0033 Tris Acetate-EDTA Buffer 50X concentrate, Reagents designed and manufactured under cGMP controls suitable for use in IVD application.
D9156 Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?