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66540013 Sigma-Aldrich

UKKi007-B

Human iPS Cell Line

Synonym: EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC

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Properties

Related Categories Cardiovascular Disorders, Cell Biology, EBiSC iPS Cells, Human iPS Cells, Stem Cell Biology More...
Quality Level   100
biological source   human dermis (fibroblast)
reprogramming method   retrovirus
description   age (45-49)
mfr. no.   EBiSC
gender   female
growth mode   adherent (pluripotent)
application(s)   cell culture | stem cell: suitable
relevant disease(s)   ventricular tachycardia
shipped in   dry ice
storage temp.   −196°C

Description

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington’s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Cell Line Origin

Depositor
Klinikum der Universität zu Köln

Cell Line Description

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Berlin, Germany
Tissue collection date: June 15, 2009
Passage number reprogrammed: P3
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Have the reprogramming vectors been silenced: Unknown
Vector map: File
Other:From the same donor: UKKi007-A

Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)TRA1-80(+)SSEA-4(+)NANOG(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:B-TUBULIN(+)FOXG1(-)HES5(+)NEUROD1(+)PAX6 (+)Sox1(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:BMP4(+)DCN(+)GATA4(-)HAND1(+)PDGF(+)PECAM (+)VIMENTIN(+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:ACTN2, cardiac alpha actinin(+)CXCR4(+)FOXA2(-)GATA6 (+)GSC (+)MLC2v, myosin light chain 2v (+)MYH6, alpha myosin heavy chain(+)PITX1 (+)RYR2 (+)SOX17(+)

Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Karyotyping method: Molecular karyotyping using using OmniExpress Exome Chip
Genotyping
Genome-wide analysis:
SNP-genotyping using OmniExpress Exome Chip
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Linkage

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Subculture Routine

Medium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°C

Legal Information

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

CRISPR Cas9 gene editing protocol for human iPSCs

Induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny arising from of all germ layers of the body including: ectoderm, endoderm, and mesoderm. The ability t...
Keywords: Alzheimer Disease, Apoptosis, Cell culture, Centrifugation, Cloning, Culture media, Gene expression, Genetic, Microscopy, Parkinson Disease, Phase transitions, Transfection

Induced Pluripotent Stem Cell Culture Protocols

Introduction Methods   Extracellular Matrix Preparation   Cell Culture Media Preparation   Thawing of Human iPSCs   Culturing of Human iPSCs   Passaging of Human iPSCs   Cryopreservation of Human iPS...
Keywords: Apoptosis, Cell culture, Cell dissociation, Cryopreservation, Culture media, Degradations, Gene expression, Immunocytochemistry, Microscopy, Phase transitions, Polymerase chain reaction, Titrations, Transcription

Related Content

The European Bank for Induced Pluripotent Stem Cells [VIDEO]

Induced pluripotent stem (iPS) cells have the potential to significantly advance drug development and medical research, yet collections of stem cells are scattered across the world, their quality can...

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