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66540486 Sigma-Aldrich

UKKi023-B

Human iPS Cell Line

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Properties

Related Categories Cardiovascular Disorders, Cell Biology, EBiSC iPS Cells, Human iPS Cells, Stem Cell Biology More...
biological source   human peripheral blood
reprogramming method   Sendai virus
description   age (50-54)
mfr. no.   EBiSC
gender   male
growth mode   adherent (pluripotent)
application(s)   cell culture | stem cell: suitable
relevant disease(s)   familial long QT syndrome
shipped in   dry ice
storage temp.   −196°C

Description

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington’s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Cell Line Origin

Depositor
Klinikum der Universität zu Köln

Cell Line Description

Derivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
FOXD3 Yes -
DNMT3b Yes -
NANOG Yes - -
SOX2 Yes -
SSEA-1 Yes -
OCT 4 Yes - - -
CD90 Yes -
TRA 1-80 Yes - -
SSEA-4 Yes - -
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:

expression of NESTIN in differentiated iPS cell line NP0126-5
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:

expression of SOX17 in differentiated iPS cell line NP0126-5
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:

expression of BRACHYURY in differentiated iPS cell line NP0126-5
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Passage number: 39
Cell line karyotype: No larger chromosomal aberrations observed
Karyotyping method: Molecular karyotyping by SNP array
Image / Result

STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Linkage

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Subculture Routine

Medium: -
Passage method: -
Matrix: -
CO2 concentration: -
O2 concentration: -
Temperature: -

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

CRISPR Cas9 gene editing protocol for human iPSCs

Induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny arising from of all germ layers of the body including: ectoderm, endoderm, and mesoderm. The ability t...
Keywords: Alzheimer Disease, Apoptosis, Cell culture, Centrifugation, Cloning, Culture media, Gene expression, Genetic, Microscopy, Parkinson Disease, Phase transitions, Transfection

Induced Pluripotent Stem Cell Culture Protocols

Introduction Methods   Extracellular Matrix Preparation   Cell Culture Media Preparation   Thawing of Human iPSCs   Culturing of Human iPSCs   Passaging of Human iPSCs   Cryopreservation of Human iPS...
Keywords: Apoptosis, Cell culture, Cell dissociation, Cryopreservation, Culture media, Degradations, Gene expression, Immunocytochemistry, Microscopy, Phase transitions, Polymerase chain reaction, Titrations, Transcription

Related Content

The European Bank for Induced Pluripotent Stem Cells [VIDEO]

Induced pluripotent stem (iPS) cells have the potential to significantly advance drug development and medical research, yet collections of stem cells are scattered across the world, their quality can...

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