66540952 Sigma-Aldrich


Human iPS Cell Line



Related Categories Cell Biology, EBiSC iPS Cells, Endocrine Disorders, Human iPS Cells, Stem Cell Biology More...
biological source   human dermis (fibroblast)
reprogramming method   Sendai virus
description   age (N/A)
mfr. no.   EBiSC
gender   female
growth mode   adherent (pluripotent)
application(s)   cell culture | stem cell: suitable
relevant disease(s)   diabetes (mellitus)
shipped in   dry ice
storage temp.   −196°C


General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington’s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Cell Line Origin


Cell Line Description

Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Is the reprogramming vector detectable: Unknown

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative

Cell line karyotype: No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array


• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Subculture Routine

Surface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: mTeSR® 1

Legal Information

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

mTeSR is a registered trademark of WiCell Research Institute, Inc.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


CRISPR Cas9 gene editing protocol for human iPSCs

Induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny arising from of all germ layers of the body including: ectoderm, endoderm, and mesoderm. The ability t...
Keywords: Alzheimer Disease, Apoptosis, Cell culture, Centrifugation, Cloning, Culture media, Gene expression, Genetic, Microscopy, Parkinson Disease, Phase transitions, Transfection

Induced Pluripotent Stem Cell Culture Protocols

Introduction Methods   Extracellular Matrix Preparation   Cell Culture Media Preparation   Thawing of Human iPSCs   Culturing of Human iPSCs   Passaging of Human iPSCs   Cryopreservation of Human iPS...
Keywords: Apoptosis, Cell culture, Cell dissociation, Cryopreservation, Culture media, Degradations, Gene expression, Immunocytochemistry, Microscopy, Phase transitions, Polymerase chain reaction, Titrations, Transcription

Related Content

The European Bank for Induced Pluripotent Stem Cells [VIDEO]

Induced pluripotent stem (iPS) cells have the potential to significantly advance drug development and medical research, yet collections of stem cells are scattered across the world, their quality can...

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