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70540 Sigma-Aldrich

Naphthol Yellow S

for microscopy (Hist.), for the precipitation (of amino acids and peptides)

Synonym: 2,4-Dinitro-1-naphthol-7-sulfonic acid sodium salt, 5,7-Dinitro-8-hydroxy-2-naphthalenesulfonic acid sodium salt, Acid Yellow 1, Flavianic acid sodium salt

  • CAS Number 846-70-8

  • Empirical Formula (Hill Notation) C10H4N2Na2O8S

  • Molecular Weight 358.19

  •  Colour Index Number 10316

  •  Beilstein/REAXYS Number 3839220

  •  EC Number 212-690-2

  •  MDL number MFCD00003958

  •  PubChem Substance ID 329762555

  •  NACRES NA.47

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Cell Biology, Hematology and Histology, Microscopy Reagents,
grade   for microscopy (Hist.)
  for the precipitation (of amino acids and peptides)
form   powder
application(s)   hematology: suitable
  histology: suitable
solubility   methanol: water (1:1): 0.5 g/10 mL, clear
εmax   ≥275 at 423-433 nm in water
  ≥≥ 250 at 387-397 nm in water
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O
InChI   1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2
InChI key   CTIQLGJVGNGFEW-UHFFFAOYSA-L

Description

Application

Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.

Biochem/physiol Actions

Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.

Analysis Note

λmax. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H2O)

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
QK1813000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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