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71196 Sigma-Aldrich

Sodium acetate solution

BioUltra, for molecular biology, ~3 M in H2O

  • CAS Number 127-09-3

  • Empirical Formula (Hill Notation) C2H3NaO2

  • Molecular Weight 82.03

  •  Beilstein/REAXYS Number 3595639

  •  MDL number MFCD00012459

  •  eCl@ss 39021906

  •  PubChem Substance ID 57652229

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Properties

Related Categories Alphabetical, BioUltra, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers,
grade   for molecular biology
InChI Key   VMHLLURERBWHNL-UHFFFAOYSA-M
product line   BioUltra
concentration   ~3 M in H2O
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
pH   7.0-8.0 (25 °C, 3 M in H2O)
anion traces   chloride (Cl-): ≤10 mg/kg
  phosphate (PO43-): ≤5 mg/kg
  sulfate (SO42-): ≤20 mg/kg
cation traces   Al: ≤1 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤1 mg/kg
  Bi: ≤1 mg/kg
  Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤50 mg/kg
  Li: ≤1 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Mo: ≤1 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Sr: ≤1 mg/kg
  Zn: ≤1 mg/kg
λ   3 M in H2O
UV absorption   λ: 260 nm Amax: ≤0.030
  λ: 280 nm Amax: ≤0.024

Description

Application

Sodium acetate solution has been used:
• in DNA extraction
• in reverse crosslinking and DNA purification
• to precipitate Gata6 (GATA binding protein 6) cDNA as a part of high-purity plasmid preparation

Biochem/physiol Actions

Sodium acetate serves as a fixed base. It is useful to rectify acidosis among acidotic patients. Orginally, sodium acetate is used as dialysate for hemodialysis, but is known to cause cardiovascular instability.

Other Notes

Basic method of DNA precipitation with ethanol

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

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Peer-Reviewed Papers
15

References

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