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71402 Sigma-Aldrich

Sodium citrate tribasic dihydrate

BioUltra, for molecular biology

Synonym: Citric acid trisodium salt dihydrate, Trisodium citrate dihydrate

  • CAS Number 6132-04-3

  • Linear Formula HOC(COONa)(CH2COONa)2 · 2H2O

  • Molecular Weight 294.10

  •  Beilstein/REAXYS Number 6104939

  •  EC Number 200-675-3

  •  MDL number MFCD00150031

  •  PubChem Substance ID 57652298

  •  NACRES NA.25

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Properties

Related Categories Alphabetical, Apoptosis and Cell Cycle, BioUltra, BioUltra Buffers, Biochemicals and Reagents,
Quality Level   200
grade   for molecular biology
product line   BioUltra
form   crystals
application(s)   precipitation assay: suitable
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
  11-13% Water (Karl Fischer)
pH   7.5-9.0 (25 °C, 0.1 M in H2O)
    (1) 3.138, (2) 4.76, (3) 6.40
mp   >300 °C (lit.)
solubility   H2O: 0.1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤10 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤100 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  NH4+: ≤10 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   0.1 M in H2O
UV absorption   λ: 260 nm Amax: 0.01
  λ: 280 nm Amax: 0.01
SMILES string   O.O.[Na+].[Na+].[Na+].OC(CC([O-])=O)(CC([O-])=O)C([O-])=O
InChI   1S/C6H8O7.3Na.2H2O/c7-3(8)1-6(13,5(11)12)2-4(9)10;;;;;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;;;2*1H2/q;3*+1;;/p-3
InChI key   NLJMYIDDQXHKNR-UHFFFAOYSA-K

Description

Other Notes

Buffer component in the preparation of total ribosomal RNA from E. coli.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

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Peer-Reviewed Papers
15

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